16S rDNA PCR analysis of infectious keratitis: a case series
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چکیده
منابع مشابه
What is broad-range 16S rDNA PCR?
Patel A, et al. Arch Dis Child Educ Pract Ed 2017;0:1–4. doi:10.1136/archdischild-2016-312049 INTRODUCTION PCRs have revolutionised the detection of bacteria in clinical samples since their widespread introduction in the 1990s. Quantitative PCR (qPCR), also known as specific PCR, involves the targeting of particular bacterial species. The technique uses specific primers (short strands of nuclei...
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Case report A 51-year-old male was admitted in Southern Railway Hospital, Chennai with complaint of abdominal pain for four days. He had a history of renal failure two years before admission and was started on CAPD for end stage renal disease. Prior to admission he was on ambulatory peritoneal dialysis (APD). Peritoneal dialysis (PD) fluid was collected and transported to laboratory aseptically...
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15 صفحه اولIdentification of acetic acid bacteria by RFLP of PCR-amplified 16S rDNA and 16S-23S rDNA intergenic spacer.
DNA corresponding to 16S rDNA and the 165-23S rDNA intergenic spacer (ITS) from 22 reference strains of acetic acid bacteria, representing the diversity of the family Acetobacteraceae, and 24 indigenous acetic acid bacteria isolated from wine fermentations were analysed by PCR-RFLP. Frateuria aurantia LMG 1558T and Escherichia coli ATCC 11775T were included as outgroups. PCR-amplified products ...
متن کاملApplicability of PCR-DGGE and 16S rDNA Sequencing for Microbiological Analysis of Otitis Media with Effusion
Background: The aim of the study was to analyze the performance of PCR-DGGE based assay and its applicability as a tool for the identification of bacteria in the middle ear of children with otitis media with effusion (OME). Methods: The middle ear effusions from 20 children with OME were analyzed both by bacterial culture and by 16S rDNA-gene-targeted PCR assay, DGGE fingerprinting and sequenci...
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ژورنال
عنوان ژورنال: Acta Ophthalmologica Scandinavica
سال: 2004
ISSN: 1395-3907,1600-0420
DOI: 10.1111/j.1395-3907.2004.00303.x